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CS0800

Sigma

 

Cathepsin D Assay Kit

sufficient for 120 assays (100 μL)


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C3138
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Cathepsin D from bovine spleen
lyophilized powder, ≥5.0 units/mg protein		C8696
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Cathepsin D from human liver
lyophilized powder, ≥250 units/mg protein (E1%/280)		M0938
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7-Methoxycoumarin-4-Acetyl-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(DNP)-D-Arg-amide
>97% (HPLC), solid		P5318


Pepstatin A
microbial

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Description

Analysis NoteThe assay is based on the hydrolysis, by the enzyme, of an internally quenched fluorimetric substrate.
Biochem/physiol ActionsCathepsin D, a ubiquitous aspartic protease belonging to the A1 peptidase family, is found intracellularly in lysosomes. The enzyme has been associated with various biological processes such as: apoptotic events (e.g., the release of cytochrome c from mitochondria and the loss of the transmembrane potential (ΔΨ), aging, Alzheimer′s disease, and breast cancer.
Features and Benefits• Contains all the reagents required for the measurement of the cathepsin D activity in cell and tissue lysates.
• The bottom limit of detection is 750 pg of protein (or 13 femtomoles of enzyme).
• A single step reaction with direct, real time observation of the degradation product released by the enzyme.
• The kit has been tested on extracts from CHO, HeLa, HEK 293, A431, HepG2, U937, and Jurkat cells on S. cerevisiae and on rat brain, kidney and liver tissues.

Properties

usage sufficient for 120 assays (100 μL)
shipped indry ice
storage temp.−20°C

Safety

Hazard CodesC
Risk Statements22-34-40
Safety Statements26-36/37/39-45
RIDADRUN 3264 8/PG 3

Components

  Kit component only
Description
Assay Buffer 1x 20 mL
Cathepsin D from bovine spleen 5 units
Albumin Solution 1 mL
Cathepsin D Substrate Solution 0.25 mL
7-Methoxycoumarin 4-acetic acid Standard Solution 0.1 mL
Pepstatin A Inhibitor Solution 0.6 mL